User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/05

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Current revision (07:54, 18 March 2013) (view source)
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==Entry title==
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==March 5, 2013==
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* Insert content here...
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=====Dilution of Molecular Beacon======
 +
*50000pM: (1μM)V=(500000pM)(1mL)
 +
25μL 1μM MB in 475μL buffer
 +
*25000pM: (50000pM)V=(25000)(40μL)
 +
20μL 50000pM in 20μL buffer
 +
*5000pM: (50000pM)V=(5000)(40μL)
 +
4μL 50000pM in 36μL buffer
 +
*500pM: (5000pM)V=(500)(40μL)
 +
4μL 5000pM in 36μL buffer
 +
*100pM: (5000pM)V=(100)(40μL)
 +
1.25μL 50000pM in 38.75μL buffer
 +
*1nm: (50000pM)V=(1000pM)(40μL)
 +
1.25μL 50000pM in 38.75μL buffer
 +
 
 +
=====Sample Data=====
 +
*1:1 1nM <br.>
 +
τ<sub>1</sub>=3.5014 ms <br.>
 +
N=9.2
 +
*1:5 1nM <br.>
 +
τ<sub>1</sub>=0.7290 ms <br.>
 +
N=10
 +
*1:10 1nM <br.>
 +
τ<sub>1</sub>=0.3791 ms <br.>
 +
N=10.9
 +
*5:1 1nM <br.>
 +
τ<sub>1</sub>=0.6540 ms <br.>
 +
N=8
 +
*10:1 1nM <br.>
 +
τ<sub>1</sub>=0.4783 ms <br.>
 +
N=14
 +
*50:1 1nM <br.>
 +
τ<sub>1</sub>=0.4286 ms <br.>
 +
N=3.5
 +
*5:1 500pM <br.>
 +
τ<sub>1</sub>=0.7030 ms <br.>
 +
N=20
 +
*10:1 500pM <br.>
 +
τ<sub>1</sub>=8.0116 ms <br.>
 +
N=7.5
 +
*50:1 500pM <br.>
 +
τ<sub>1</sub>=90515.3 ms <br.>
 +
N=1.91
 +
 

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March 5, 2013

Dilution of Molecular Beacon=
  • 50000pM: (1μM)V=(500000pM)(1mL)

25μL 1μM MB in 475μL buffer

  • 25000pM: (50000pM)V=(25000)(40μL)

20μL 50000pM in 20μL buffer

  • 5000pM: (50000pM)V=(5000)(40μL)

4μL 50000pM in 36μL buffer

  • 500pM: (5000pM)V=(500)(40μL)

4μL 5000pM in 36μL buffer

  • 100pM: (5000pM)V=(100)(40μL)

1.25μL 50000pM in 38.75μL buffer

  • 1nm: (50000pM)V=(1000pM)(40μL)

1.25μL 50000pM in 38.75μL buffer

Sample Data
  • 1:1 1nM

τ1=3.5014 ms
N=9.2

  • 1:5 1nM

τ1=0.7290 ms
N=10

  • 1:10 1nM

τ1=0.3791 ms
N=10.9

  • 5:1 1nM

τ1=0.6540 ms
N=8

  • 10:1 1nM

τ1=0.4783 ms
N=14

  • 50:1 1nM

τ1=0.4286 ms
N=3.5

  • 5:1 500pM

τ1=0.7030 ms
N=20

  • 10:1 500pM

τ1=8.0116 ms
N=7.5

  • 50:1 500pM

τ1=90515.3 ms
N=1.91



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