User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/05: Difference between revisions
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== | ==March 5, 2013== | ||
* | =====Dilution of Molecular Beacon====== | ||
*50000pM: (1μM)V=(500000pM)(1mL) | |||
25μL 1μM MB in 475μL buffer | |||
*25000pM: (50000pM)V=(25000)(40μL) | |||
20μL 50000pM in 20μL buffer | |||
*5000pM: (50000pM)V=(5000)(40μL) | |||
4μL 50000pM in 36μL buffer | |||
*500pM: (5000pM)V=(500)(40μL) | |||
4μL 5000pM in 36μL buffer | |||
*100pM: (5000pM)V=(100)(40μL) | |||
1.25μL 50000pM in 38.75μL buffer | |||
*1nm: (50000pM)V=(1000pM)(40μL) | |||
1.25μL 50000pM in 38.75μL buffer | |||
=====Sample Data===== | |||
*1:1 1nM <br.> | |||
τ<sub>1</sub>=3.5014 ms <br.> | |||
N=9.2 | |||
*1:5 1nM <br.> | |||
τ<sub>1</sub>=0.7290 ms <br.> | |||
N=10 | |||
*1:10 1nM <br.> | |||
τ<sub>1</sub>=0.3791 ms <br.> | |||
N=10.9 | |||
*5:1 1nM <br.> | |||
τ<sub>1</sub>=0.6540 ms <br.> | |||
N=8 | |||
*10:1 1nM <br.> | |||
τ<sub>1</sub>=0.4783 ms <br.> | |||
N=14 | |||
*50:1 1nM <br.> | |||
τ<sub>1</sub>=0.4286 ms <br.> | |||
N=3.5 | |||
*5:1 500pM <br.> | |||
τ<sub>1</sub>=0.7030 ms <br.> | |||
N=20 | |||
*10:1 500pM <br.> | |||
τ<sub>1</sub>=8.0116 ms <br.> | |||
N=7.5 | |||
*50:1 500pM <br.> | |||
τ<sub>1</sub>=90515.3 ms <br.> | |||
N=1.91 | |||
Revision as of 05:54, 18 March 2013
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March 5, 2013Dilution of Molecular Beacon=
25μL 1μM MB in 475μL buffer
20μL 50000pM in 20μL buffer
4μL 50000pM in 36μL buffer
4μL 5000pM in 36μL buffer
1.25μL 50000pM in 38.75μL buffer
1.25μL 50000pM in 38.75μL buffer Sample Data
τ1=3.5014 ms <br.> N=9.2
τ1=0.7290 ms <br.> N=10
τ1=0.3791 ms <br.> N=10.9
τ1=0.6540 ms <br.> N=8
τ1=0.4783 ms <br.> N=14
τ1=0.4286 ms <br.> N=3.5
τ1=0.7030 ms <br.> N=20
τ1=8.0116 ms <br.> N=7.5
τ1=90515.3 ms <br.> N=1.91
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