User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/05

March 5, 2013

Dilution of Molecular Beacon=

• 50000pM: (1μM)V=(500000pM)(1mL)

25μL 1μM MB in 475μL buffer

• 25000pM: (50000pM)V=(25000)(40μL)

20μL 50000pM in 20μL buffer

• 5000pM: (50000pM)V=(5000)(40μL)

4μL 50000pM in 36μL buffer

• 500pM: (5000pM)V=(500)(40μL)

4μL 5000pM in 36μL buffer

• 100pM: (5000pM)V=(100)(40μL)

1.25μL 50000pM in 38.75μL buffer

• 1nm: (50000pM)V=(1000pM)(40μL)

1.25μL 50000pM in 38.75μL buffer

Sample Data

• 1:1 1nM
  

τ₁=3.5014 ms
N=9.2

• 1:5 1nM
  

τ₁=0.7290 ms
N=10

• 1:10 1nM
  

τ₁=0.3791 ms
N=10.9

• 5:1 1nM
  

τ₁=0.6540 ms
N=8

• 10:1 1nM
  

τ₁=0.4783 ms
N=14

• 50:1 1nM
  

τ₁=0.4286 ms
N=3.5

• 5:1 500pM
  

τ₁=0.7030 ms
N=20

• 10:1 500pM
  

τ₁=8.0116 ms
N=7.5

• 50:1 500pM
  

τ₁=90515.3 ms
N=1.91