User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26

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(February 26, 2013)
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==February 26, 2013==
==February 26, 2013==
-
* Hybridization of molecular beacon and complementary strand at varying concentrations
+
===Hybridization of molecular beacon and complementary strand at varying concentrations===
-
===MB:CS (MB at 500pM)===
+
====MB:CS (MB at 500pM)====
-
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
+
(7.3x10^-6M)V=(3x10^-6)(10μL)
(7.3x10^-6M)V=(3x10^-6)(10μL)
4.1μL MB in 5.9μL buffer
4.1μL MB in 5.9μL buffer
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<br.>
<br.>
(3x10^-6)V=(500x10^-9)(50μL)
(3x10^-6)V=(500x10^-9)(50μL)
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8.33μL MB in 41.67μL buffer
+
8.33μL in 41.67μL  
 +
<br.>
 +
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS
<br.>
<br.>
-
===MB:CS (CS at 500pM)===
+
====MB:CS (CS at 500pM)====
 +
(7.3x10^-6)V=(5x10^-6)(5μL)
 +
3.42μL MB in 1.57μL buffer
 +
<br.>
 +
0.36μL CS in 9.64μL buffer
 +
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
 +
*5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS
 +
*10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS
 +
*50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS
 +
<br.>
 +
Make 1nm, 500pM, and 100pM samples for each ratio

Revision as of 21:28, 17 March 2013

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February 26, 2013

Hybridization of molecular beacon and complementary strand at varying concentrations

MB:CS (MB at 500pM)

(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer
(2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer
(3x10^-6)V=(500x10^-9)(50μL) 8.33μL in 41.67μL

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
  • 1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
  • 1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS


MB:CS (CS at 500pM)

(7.3x10^-6)V=(5x10^-6)(5μL) 3.42μL MB in 1.57μL buffer
0.36μL CS in 9.64μL buffer

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
  • 5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS
  • 10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS
  • 50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS


Make 1nm, 500pM, and 100pM samples for each ratio



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