User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26

February 26, 2013

• Hybridization of molecular beacon and complementary strand at varying concentrations

MB:CS (MB at 500pM)

• 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS

(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer
(2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer
(3x10^-6)V=(500x10^-9)(50μL) 8.33μL MB in 41.67μL buffer

• 1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
• 1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS

MB:CS (CS at 500pM)