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 Revision as of 21:20, 17 March 2013 (view source) (→Entry title)← Previous diff Revision as of 21:28, 17 March 2013 (view source) (→February 26, 2013)Next diff → Line 7: Line 7: ==February 26, 2013== ==February 26, 2013== - * Hybridization of molecular beacon and complementary strand at varying concentrations + ===Hybridization of molecular beacon and complementary strand at varying concentrations=== - ===MB:CS (MB at 500pM)=== + ====MB:CS (MB at 500pM)==== - *1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS + (7.3x10^-6M)V=(3x10^-6)(10μL) (7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer 4.1μL MB in 5.9μL buffer Line 17: Line 16: (3x10^-6)V=(500x10^-9)(50μL) (3x10^-6)V=(500x10^-9)(50μL) - 8.33μL MB in 41.67μL buffer + 8.33μL in 41.67μL + + *1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS *1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS *1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS *1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS *1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS - ===MB:CS (CS at 500pM)=== + ====MB:CS (CS at 500pM)==== + (7.3x10^-6)V=(5x10^-6)(5μL) + 3.42μL MB in 1.57μL buffer + + 0.36μL CS in 9.64μL buffer + *1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS + *5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS + *10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS + *50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS + + Make 1nm, 500pM, and 100pM samples for each ratio

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February 26, 2013

Hybridization of molecular beacon and complementary strand at varying concentrations

MB:CS (MB at 500pM)

(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer
(2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer
(3x10^-6)V=(500x10^-9)(50μL) 8.33μL in 41.67μL

• 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
• 1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
• 1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS

MB:CS (CS at 500pM)

(7.3x10^-6)V=(5x10^-6)(5μL) 3.42μL MB in 1.57μL buffer
0.36μL CS in 9.64μL buffer

• 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
• 5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS
• 10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS
• 50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS

Make 1nm, 500pM, and 100pM samples for each ratio