User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26: Difference between revisions
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==February 26, 2013== | ==February 26, 2013== | ||
===Hybridization of molecular beacon and complementary strand at varying concentrations=== | |||
===MB:CS (MB at 500pM)=== | ====MB:CS (MB at 500pM)==== | ||
(7.3x10^-6M)V=(3x10^-6)(10μL) | (7.3x10^-6M)V=(3x10^-6)(10μL) | ||
4.1μL MB in 5.9μL buffer | 4.1μL MB in 5.9μL buffer | ||
Line 17: | Line 16: | ||
<br.> | <br.> | ||
(3x10^-6)V=(500x10^-9)(50μL) | (3x10^-6)V=(500x10^-9)(50μL) | ||
8.33μL | 8.33μL in 41.67μL | ||
<br.> | |||
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS | |||
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS | *1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS | ||
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS | *1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS | ||
<br.> | <br.> | ||
===MB:CS (CS at 500pM)=== | ====MB:CS (CS at 500pM)==== | ||
(7.3x10^-6)V=(5x10^-6)(5μL) | |||
3.42μL MB in 1.57μL buffer | |||
<br.> | |||
0.36μL CS in 9.64μL buffer | |||
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS | |||
*5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS | |||
*10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS | |||
*50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS | |||
<br.> | |||
Make 1nm, 500pM, and 100pM samples for each ratio | |||
Revision as of 18:28, 17 March 2013
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February 26, 2013Hybridization of molecular beacon and complementary strand at varying concentrationsMB:CS (MB at 500pM)(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer <br.> (2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer <br.> (3x10^-6)V=(500x10^-9)(50μL) 8.33μL in 41.67μL <br.>
<br.> MB:CS (CS at 500pM)(7.3x10^-6)V=(5x10^-6)(5μL) 3.42μL MB in 1.57μL buffer <br.> 0.36μL CS in 9.64μL buffer
<br.> Make 1nm, 500pM, and 100pM samples for each ratio
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