User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/02/26 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
(Entry title)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==February 26, 2013==
-
* Insert content here...
+
* Hybridization of molecular beacon and complementary strand at varying concentrations
 +
===MB:CS (MB at 500pM)===
 +
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
 +
(7.3x10^-6M)V=(3x10^-6)(10μL)
 +
4.1μL MB in 5.9μL buffer
 +
<br.>
 +
(2.8x10^-5)V=(3x10^-6M)(10μL)
 +
1.1μL CS in 8.9μL buffer
 +
<br.>
 +
(3x10^-6)V=(500x10^-9)(50μL)
 +
8.33μL MB in 41.67μL buffer
 +
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
 +
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS
 +
<br.>
 +
===MB:CS (CS at 500pM)===
 +
 

Revision as of 21:20, 17 March 2013

Project name Main project page
Previous entry      Next entry

February 26, 2013

  • Hybridization of molecular beacon and complementary strand at varying concentrations

MB:CS (MB at 500pM)

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS

(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer
(2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer
(3x10^-6)V=(500x10^-9)(50μL) 8.33μL MB in 41.67μL buffer

  • 1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
  • 1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS


MB:CS (CS at 500pM)


Personal tools