User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26
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| - | == | + | ==February 26, 2013== |
| - | * | + | * Hybridization of molecular beacon and complementary strand at varying concentrations |
| + | ===MB:CS (MB at 500pM)=== | ||
| + | *1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS | ||
| + | (7.3x10^-6M)V=(3x10^-6)(10μL) | ||
| + | 4.1μL MB in 5.9μL buffer | ||
| + | <br.> | ||
| + | (2.8x10^-5)V=(3x10^-6M)(10μL) | ||
| + | 1.1μL CS in 8.9μL buffer | ||
| + | <br.> | ||
| + | (3x10^-6)V=(500x10^-9)(50μL) | ||
| + | 8.33μL MB in 41.67μL buffer | ||
| + | *1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS | ||
| + | *1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS | ||
| + | <br.> | ||
| + | ===MB:CS (CS at 500pM)=== | ||
| + | |||
Revision as of 21:20, 17 March 2013
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February 26, 2013
MB:CS (MB at 500pM)
(7.3x10^-6M)V=(3x10^-6)(10μL)
4.1μL MB in 5.9μL buffer
MB:CS (CS at 500pM) | |
