User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26

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(Autocreate 2013/02/26 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
Current revision (07:34, 18 March 2013) (view source)
(February 26, 2013)
 
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==Entry title==
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==February 26, 2013==
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* Insert content here...
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===Hybridization of molecular beacon and complementary strand at varying concentrations===
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====MB:CS (MB at 500pM)====
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(7.3x10^-6M)V=(3x10^-6)(10μL)<br.>
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4.1μL MB in 5.9μL buffer
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<br.>
 +
(2.8x10^-5)V=(3x10^-6M)(10μL)<br.>
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1.1μL CS in 8.9μL buffer
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<br.>
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(3x10^-6)V=(500x10^-9)(50μL)<br.>
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8.33μL in 41.67μL
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<br.>
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*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
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*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
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*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS
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<br.>
 +
====MB:CS (CS at 500pM)====
 +
(7.3x10^-6)V=(5x10^-6)(5μL)
 +
3.42μL MB in 1.57μL buffer
 +
<br.>
 +
0.36μL CS in 9.64μL buffer
 +
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
 +
*5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS
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*10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS
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*50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS
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<br.>
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=====Make 1nm, 500pM, and 100pM samples for each ratio=====
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1:1
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*50nM: (500nM)V=(50nM)(50μL)
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5μL 500nM solution in 45μL buffer
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*1nM: (50nM)V=(1nM)(50μL)
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1μL 50nM solution in 49μL buffer
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*500pM (0.5nM): (1nM)V=(0.5nM)(50μL)
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25μL 1nM solution in 25μL buffer
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*100pM (0.1nM): (0.5nM)V=(0.1nM)(50μL)
 +
10μL 0.5nM solution in 40μL buffer
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1:5, 1:10, 5:1, 10:1, and 50:1 calculations done in the same way
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February 26, 2013

Hybridization of molecular beacon and complementary strand at varying concentrations

MB:CS (MB at 500pM)

(7.3x10^-6M)V=(3x10^-6)(10μL)
4.1μL MB in 5.9μL buffer
(2.8x10^-5)V=(3x10^-6M)(10μL)
1.1μL CS in 8.9μL buffer
(3x10^-6)V=(500x10^-9)(50μL)
8.33μL in 41.67μL

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
  • 1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
  • 1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS


MB:CS (CS at 500pM)

(7.3x10^-6)V=(5x10^-6)(5μL) 3.42μL MB in 1.57μL buffer
0.36μL CS in 9.64μL buffer

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
  • 5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS
  • 10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS
  • 50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS


Make 1nm, 500pM, and 100pM samples for each ratio

1:1

  • 50nM: (500nM)V=(50nM)(50μL)

5μL 500nM solution in 45μL buffer

  • 1nM: (50nM)V=(1nM)(50μL)

1μL 50nM solution in 49μL buffer

  • 500pM (0.5nM): (1nM)V=(0.5nM)(50μL)

25μL 1nM solution in 25μL buffer

  • 100pM (0.1nM): (0.5nM)V=(0.1nM)(50μL)

10μL 0.5nM solution in 40μL buffer 1:5, 1:10, 5:1, 10:1, and 50:1 calculations done in the same way



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