User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/19
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#2.0μM 4 Strand DNA with 3.5μM ThT | #2.0μM 4 Strand DNA with 3.5μM ThT | ||
#2.5μM 8 Strand DNA with 3.5μM ThT | #2.5μM 8 Strand DNA with 3.5μM ThT | ||
| + | Solutions were heated at approximately 70°C for 25 minutes | ||
*Hybridize:DNA and ZnPPIX solutions using same method as ThT | *Hybridize:DNA and ZnPPIX solutions using same method as ThT | ||
| + | example: | ||
| + | (265.6μM 8 Strand DNA)(v)=(3.5μM 8 Strand DNA)(250μL) | ||
| + | v=3.29μL of 265.6μM 8 Strand DNA in 246.71μL TRIS buffer solution | ||
| + | combined with 250μL 5μM ZnPPIX, solution is heated for hybridization | ||
#3.5μM 4 Strand DNA with 5μM ZnPPIX | #3.5μM 4 Strand DNA with 5μM ZnPPIX | ||
#3.0μM 4 Strand DNA with 5μM ZnPPIX | #3.0μM 4 Strand DNA with 5μM ZnPPIX | ||
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#2.0μM 8 Strand DNA with 5μM ZnPPIX | #2.0μM 8 Strand DNA with 5μM ZnPPIX | ||
#1.5μM 8 Strand DNA with 5μM ZnPPIX | #1.5μM 8 Strand DNA with 5μM ZnPPIX | ||
| + | Solutions were heated at approximately 70°C for 25 minutes | ||
Revision as of 14:11, 19 February 2013
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February 19, 2013
Solutions were heated at approximately 70°C for 25 minutes
example: (265.6μM 8 Strand DNA)(v)=(3.5μM 8 Strand DNA)(250μL) v=3.29μL of 265.6μM 8 Strand DNA in 246.71μL TRIS buffer solution combined with 250μL 5μM ZnPPIX, solution is heated for hybridization
Solutions were heated at approximately 70°C for 25 minutes
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