User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/05

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(February 5, 2013)
Current revision (12:30, 12 February 2013) (view source)
(February 5, 2013)
 
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#1.5μM 8 strand DNA in 3.5μM ThT
#1.5μM 8 strand DNA in 3.5μM ThT
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*4 strand DNA in 3.5μM ThT
+
*Absorbance spectra for varying concentrations of 4 strand DNA in 3.5μM ThT
[[Image:4repeat_DNA_and_ThT_graph.jpg]]
[[Image:4repeat_DNA_and_ThT_graph.jpg]]
 +
 +
*Absorbance spectra for varying concentrations of 8 strand DNA in 3.5μM ThT
 +
[[Image:8repeat_DNA_and_ThT_graph.jpg]]

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February 5, 2013

  • Absorbance spectra were taken for the following solutions:
  1. 6.49nM Molecular Beacon
  2. 3.5μM ThT
  3. 5μM ZnPPIX
  4. 3.5μM ThT and 3.5μM ZnPPIX
  5. 1μM 4 strand DNA
  6. 1μM 8 strand DNA
  7. ZnPPIX and 4 strand DNA
  8. ZnPPIX and 8 strand DNA
  9. 3.5μM 4 strand DNA in 3.5μM ThT
  10. 3.0μM 4 strand DNA in 3.5μM ThT
  11. 2.5μM 4 strand DNA in 3.5μM ThT
  12. 2.0μM 4 strand DNA in 3.5μM ThT
  13. 1.5μM 4 strand DNA in 3.5μM ThT
  14. 3.5μM 8 strand DNA in 3.5μM ThT
  15. 3.0μM 8 strand DNA in 3.5μM ThT
  16. 2.5μM 8 strand DNA in 3.5μM ThT
  17. 2.0μM 8 strand DNA in 3.5μM ThT
  18. 1.5μM 8 strand DNA in 3.5μM ThT
  • Absorbance spectra for varying concentrations of 4 strand DNA in 3.5μM ThT

Image:4repeat_DNA_and_ThT_graph.jpg

  • Absorbance spectra for varying concentrations of 8 strand DNA in 3.5μM ThT

Image:8repeat_DNA_and_ThT_graph.jpg



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