User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/05
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| - | == | + | ==February 5, 2013== |
| - | * | + | * Absorbance spectra were taken for the following solutions: |
| + | #6.49nM Molecular Beacon | ||
| + | #3.5μM ThT | ||
| + | #5μM ZnPPIX | ||
| + | #3.5μM ThT and 3.5μM ZnPPIX | ||
| + | #1μM 4 strand DNA | ||
| + | #1μM 8 strand DNA | ||
| + | #ZnPPIX and 4 strand DNA | ||
| + | #ZnPPIX and 8 strand DNA | ||
| + | #3.5μM 4 strand DNA in 3.5μM ThT | ||
| + | #3.0μM 4 strand DNA in 3.5μM ThT | ||
| + | #2.5μM 4 strand DNA in 3.5μM ThT | ||
| + | #2.0μM 4 strand DNA in 3.5μM ThT | ||
| + | #1.5μM 4 strand DNA in 3.5μM ThT | ||
| + | #3.5μM 8 strand DNA in 3.5μM ThT | ||
| + | #3.0μM 8 strand DNA in 3.5μM ThT | ||
| + | #2.5μM 8 strand DNA in 3.5μM ThT | ||
| + | #2.0μM 8 strand DNA in 3.5μM ThT | ||
| + | #1.5μM 8 strand DNA in 3.5μM ThT | ||
| + | |||
| + | *Absorbance spectra for varying concentrations of 4 strand DNA in 3.5μM ThT | ||
| + | [[Image:4repeat_DNA_and_ThT_graph.jpg]] | ||
| + | |||
| + | *Absorbance spectra for varying concentrations of 8 strand DNA in 3.5μM ThT | ||
| + | [[Image:8repeat_DNA_and_ThT_graph.jpg]] | ||
| + | |||
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