User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/05

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(Autocreate 2013/02/05 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
Current revision (12:30, 12 February 2013) (view source)
(February 5, 2013)
 
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==Entry title==
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==February 5, 2013==
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* Insert content here...
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* Absorbance spectra were taken for the following solutions:
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#6.49nM Molecular Beacon
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#3.5μM ThT
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#5μM ZnPPIX
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#3.5μM ThT and 3.5μM ZnPPIX
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#1μM 4 strand DNA
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#1μM 8 strand DNA
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#ZnPPIX and 4 strand DNA
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#ZnPPIX and 8 strand DNA
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#3.5μM 4 strand DNA in 3.5μM ThT
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#3.0μM 4 strand DNA in 3.5μM ThT
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#2.5μM 4 strand DNA in 3.5μM ThT
 +
#2.0μM 4 strand DNA in 3.5μM ThT
 +
#1.5μM 4 strand DNA in 3.5μM ThT
 +
#3.5μM 8 strand DNA in 3.5μM ThT
 +
#3.0μM 8 strand DNA in 3.5μM ThT
 +
#2.5μM 8 strand DNA in 3.5μM ThT
 +
#2.0μM 8 strand DNA in 3.5μM ThT
 +
#1.5μM 8 strand DNA in 3.5μM ThT
 +
 
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*Absorbance spectra for varying concentrations of 4 strand DNA in 3.5μM ThT
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[[Image:4repeat_DNA_and_ThT_graph.jpg]]
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*Absorbance spectra for varying concentrations of 8 strand DNA in 3.5μM ThT
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[[Image:8repeat_DNA_and_ThT_graph.jpg]]
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February 5, 2013

  • Absorbance spectra were taken for the following solutions:
  1. 6.49nM Molecular Beacon
  2. 3.5μM ThT
  3. 5μM ZnPPIX
  4. 3.5μM ThT and 3.5μM ZnPPIX
  5. 1μM 4 strand DNA
  6. 1μM 8 strand DNA
  7. ZnPPIX and 4 strand DNA
  8. ZnPPIX and 8 strand DNA
  9. 3.5μM 4 strand DNA in 3.5μM ThT
  10. 3.0μM 4 strand DNA in 3.5μM ThT
  11. 2.5μM 4 strand DNA in 3.5μM ThT
  12. 2.0μM 4 strand DNA in 3.5μM ThT
  13. 1.5μM 4 strand DNA in 3.5μM ThT
  14. 3.5μM 8 strand DNA in 3.5μM ThT
  15. 3.0μM 8 strand DNA in 3.5μM ThT
  16. 2.5μM 8 strand DNA in 3.5μM ThT
  17. 2.0μM 8 strand DNA in 3.5μM ThT
  18. 1.5μM 8 strand DNA in 3.5μM ThT
  • Absorbance spectra for varying concentrations of 4 strand DNA in 3.5μM ThT

Image:4repeat_DNA_and_ThT_graph.jpg

  • Absorbance spectra for varying concentrations of 8 strand DNA in 3.5μM ThT

Image:8repeat_DNA_and_ThT_graph.jpg



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