User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/01/29: Difference between revisions
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5) make 3.5μM ThT <br> | 5) make 3.5μM ThT <br> | ||
(318.86g/mol ThT)(3.5mmol/L)(1mol/10^3mmol)=1.116g/L(1L/1000mL)(10mL)=0.01116g ThT in 10mL solution <br> | (318.86g/mol ThT)(3.5mmol/L)(1mol/10^3mmol)=1.116g/L(1L/1000mL)(10mL)=0.01116g ThT in 10mL solution <br> | ||
*dissolve 0.0134g ThT in 10mL filtered H<sub>2</sub>O : 3.5mM ThT solution <br> | *dissolve 0.0134g ThT (massed value, approximated for 0.01116g) in 10mL filtered H<sub>2</sub>O : 3.5mM ThT solution <br> | ||
*dilute 10μL 3.5mM ThT solution in 9990μL filtered H<sub>2</sub>O : 3.5μM ThT solution <br> | *dilute 10μL 3.5mM ThT solution in 9990μL filtered H<sub>2</sub>O : 3.5μM ThT solution <br> | ||
6) make DNA solutions at varying concentrations: <br> | 6) make DNA solutions at varying concentrations: <br> |
Revision as of 08:45, 2 April 2013
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January 29, 2013
1) make 100μL of 1μM 4 strand DNA:
5) make 3.5μM ThT
6) make DNA solutions at varying concentrations:
V=3.29μL 4 strand DNA in 246.71μL TRIS buffer solution
V=6.13μL 8 strand DNA in 243.87μL TRIS buffer solution
7)mix 100μL of each of the 10 DNA solutions with 100μL 3.5μM ThT
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