User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/01/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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5) make 3.5μM ThT <br> | 5) make 3.5μM ThT <br> | ||
(318.86g/mol ThT)(3.5mmol/L)(1mol/10^3mmol)=1.116g/L(1L/1000mL)(10mL)=0.01116g ThT in 10mL solution <br> | (318.86g/mol ThT)(3.5mmol/L)(1mol/10^3mmol)=1.116g/L(1L/1000mL)(10mL)=0.01116g ThT in 10mL solution <br> | ||
*dissolve 0.0134g ThT in 10mL filtered H<sub>2</sub>O : 3.5mM ThT solution <br> | *dissolve 0.0134g ThT (massed value, approximated for 0.01116g) in 10mL filtered H<sub>2</sub>O : 3.5mM ThT solution <br> | ||
*dilute 10μL 3.5mM ThT solution in 9990μL filtered H<sub>2</sub>O : 3.5μM ThT solution <br> | *dilute 10μL 3.5mM ThT solution in 9990μL filtered H<sub>2</sub>O : 3.5μM ThT solution <br> | ||
6) make DNA solutions at varying concentrations: <br> | 6) make DNA solutions at varying concentrations: <br> |
Latest revision as of 22:25, 26 September 2017
Project name | Main project page Next entry |
January 29, 2013
1) make 100μL of 1μM 4 strand DNA:
5) make 3.5μM ThT
6) make DNA solutions at varying concentrations:
V=3.29μL 4 strand DNA in 246.71μL TRIS buffer solution
V=6.13μL 8 strand DNA in 243.87μL TRIS buffer solution
7)mix 100μL of each of the 10 DNA solutions with 100μL 3.5μM ThT
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