User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/02/27: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 20: Line 20:
# Prepare 210 mL of citric acid, calcium chloride solution
# Prepare 210 mL of citric acid, calcium chloride solution
## Add '''12-15 g (5-7%) citric acid'''  
## Add '''12-15 g (5-7%) citric acid'''  
## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water
## Add '''8.4 g (4%) calcium chloride'''
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
## Heat solution to 40°C while stirring for 30 min
## Heat solution to 40°C while stirring for 30 min

Revision as of 09:19, 25 February 2015

PVA-Alg Bead Synthesis <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 27, 2015

  • Heat citric acid/CaCl2 solution and use Dr Hartings' smaller syringe tips

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of citric acid, calcium chloride solution
    1. Add 12-15 g (5-7%) citric acid
    2. Add 8.4 g (4%) calcium chloride
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
    1. Heat solution to 40°C while stirring for 30 min
    2. Allow solution to cool to room temp while stirring for another 20 min
  5. Leave beads in solution, not stirring, overnight
  6. Stir in 10% citric acid solution for 30 min
  7. Stir in 0.5 M sodium sulfate solution for 30 min
  8. Rinse with water to remove excess reactants
  9. Allow beads to dry in teflon plate overnight

Measurements

Notes


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/CHEM-582_Experimental_Chemistry_II/2015/02/27&oldid=867936"