User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/02/27: Difference between revisions

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===Procedure===
===Procedure===
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.>
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.>
<br.>
<br.>
PVA-Alg-NaMT (no citric acid)
PVA-Alg-NaMT (no citric acid)
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# Prepare 210 mL of saturated citric acid with 4% calcium chloride
# Prepare 210 mL of saturated citric acid with 4% calcium chloride
## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water
## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
## Heat solution to 40°C while stirring for 30 min
## Heat solution to 40°C while stirring for 30 min
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
## Allow solution to cool to room temp while stirring for another 20 min
## Allow solution to cool to room temp while stirring for another 20 min
#Leave beads in solution, not stirring, overnight
#Leave beads in solution, not stirring, overnight

Revision as of 08:45, 25 February 2015

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February 27, 2015

  • Heat citric acid/CaCl2 solution and use Dr Hartings' smaller syringe tips

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT (no citric acid)

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 4% calcium chloride
    1. Add 8.4 g (4%) calcium chloride to 210 mL of water
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
    1. Heat solution to 40°C while stirring for 30 min
    2. Allow solution to cool to room temp while stirring for another 20 min
  5. Leave beads in solution, not stirring, overnight
  6. Stir in 10% citric acid solution for 30 min
  7. Stir in 0.5 M sodium sulfate solution for 30 min
  8. Rinse with water to remove excess reactants
  9. Allow beads to dry in teflon plate overnight

Measurements

Notes


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