User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/02/27: Difference between revisions

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#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate)  
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate)  
##* Add alginate '''while stirring''' PVA-water solution
##* Add alginate '''while stirring''' PVA-water solution
#* '''~0.1 g of NaMT''' and '''~0.05 g AC''' should be added to this mixture where applicable
#* '''~0.1 g of NaMT''' should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of saturated citric acid with 4% calcium chloride
# Prepare 210 mL of saturated citric acid with 4% calcium chloride

Revision as of 08:13, 25 February 2015

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February 27, 2015

  • Heat citric acid/CaCl2 solution and use Dr Hartings' smaller syringe tips

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.> <br.> PVA-Alg-NaMT (no citric acid)

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 4% calcium chloride
    1. Add 8.4 g (4%) calcium chloride to 210 mL of water
    2. Heat solution to 40°C
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
  5. Stir for 2 hrs to allow solidification
  6. Rinse with water to remove excess reactants

Measurements

Notes


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