User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/01/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==January 30, 2015== | ==January 30, 2015== | ||
*Malachite Green calibration curve | *Malachite Green calibration curve | ||
*redo PVA-Alg bead synthesis | *redo PVA-Alg/PVA-Alg-NaMT bead synthesis, same scale, 4% CaCl, smaller beads using glass pipette | ||
===Procedure=== | ===Procedure=== | ||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | |||
Modified from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-582_Experimental_Chemistry_II/2015/01/28 1/28/2015] | |||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | |||
#* 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) | |||
##* Add alginate '''while stirring''' PVA-water solution | |||
#* ~0.1 g of nanocomposites should be added to this mixture where applicable | |||
# Cool dissolved and mixed solution to 35°C | |||
# Prepare 210 mL of saturated citric acid with 2% calcium chloride | |||
## Add 8.4 grams (4%) of calcium chloride to 210 mL of water | |||
## Add citric acid to saturation (32 g citric acid) | |||
##* 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid | |||
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette | |||
# Stir for 2 hrs to allow solidification | |||
# Rinse with water to remove excess reactants | |||
====Measurements==== | |||
*PVA-Alg | |||
**PVA: 1.044 g | |||
**Alginate: 0.16 g | |||
**CaCl: 8.213 g | |||
**citric acid: 32.01 g | |||
*PVA-Alg-NaMT | |||
**PVA: 0.994 g | |||
**Alg: 0.156 g | |||
**NaMT: 0.103 g | |||
**CaCl: 8.381 g | |||
**citric acid: 33.01 g | |||
*PVA-Alg-NaMT #2 | |||
**PVA: 1.098 g | |||
**Alginate: 0.159 g | |||
**NaMT: 0.125 g | |||
**CaCl: 8.9 g | |||
**citric acid: 32.02 g | |||
**CaCl: 8.403 g | |||
**citric acid: 32.04 g | |||
*Vial, no cap: 13.023 g | |||
====Notes==== | ====Notes==== | ||
*PVA-Alg does not work | |||
*PVA-Alg-NaMT #2 made in sonicator | |||
====Next Week==== | |||
*Madeleine: MG Calibration curve | |||
*Eleni: Scale up PVA-Alg-NaMT synthesis | |||
*James: start next nanocomposite synthesis | |||
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Latest revision as of 00:41, 27 September 2017
PVA-Alg Bead Synthesis | Main project page Previous entry Next entry |
January 30, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.> Modified from 1/28/2015
Measurements
Notes
Next Week
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