User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/01/28: Difference between revisions
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===Procedure=== | ===Procedure=== | ||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | ||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C | # Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | ||
# | #* 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) | ||
##* Add alginate '''while stirring''' PVA-water solution | ##* Add alginate '''while stirring''' PVA-water solution | ||
# | #* ~0.1 g of nanocomposites should be added to this mixture where applicable | ||
# Cool dissolved and mixed solution to 35°C | # Cool dissolved and mixed solution to 35°C | ||
# Prepare 210 mL of saturated citric acid with 2% calcium chloride | # Prepare 210 mL of saturated citric acid with 2% calcium chloride | ||
## Add 4.2 grams of calcium chloride to 210 mL of water | ## Add 4.2 grams of calcium chloride to 210 mL of water | ||
## Add citric acid to saturation | ## Add citric acid to saturation (32 g citric acid) | ||
# Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle | ##* 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid | ||
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a thin needle | |||
# Stir for 24hrs to allow solidification | # Stir for 24hrs to allow solidification | ||
# Rinse with water to remove excess reactants | # Rinse with water to remove excess reactants | ||
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**0.161 g alginate | **0.161 g alginate | ||
**0.109 g NaMT | **0.109 g NaMT | ||
*Saturated citric acid, 2% calcium chloride solution | |||
**4.224 g calcium chloride | |||
**32.363 g citric acid | |||
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Revision as of 07:35, 28 January 2015
 PVA-Alg Bead Synthesis
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January, 27, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>
Measurements
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