January, 27, 2015
- First trial of synthesizing PVA-Alginate beads with and without NaMT
Procedure
Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>
- Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
- 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
- Add alginate while stirring PVA-water solution
- ~0.1 g of nanocomposites should be added to this mixture where applicable
- Cool dissolved and mixed solution to 35°C
- Prepare 210 mL of saturated citric acid with 2% calcium chloride
- Add 4.2 grams of calcium chloride to 210 mL of water
- Add citric acid to saturation (32 g citric acid)
- 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid
- Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a thin needle
- Stir for 24hrs to allow solidification
- Rinse with water to remove excess reactants
Measurements
- PVA-Alg
- 1.015 g PVA
- 0.1649 g alginate
- PVA-Alg-NaMT
- 0.999 g PVA
- 0.161 g alginate
- 0.109 g NaMT
- Saturated citric acid, 2% calcium chloride solution
- 4.224 g calcium chloride
- 32.363 g citric acid
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