January, 27, 2015
- First trial of synthesizing PVA-Alginate beads with and without NaMT
Protocol
Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>
- Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C
- 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
- ~0.1 g of nanocomposites should be added to this mixture where applicable
- Cool dissolved and mixed solution to 35°C
- Prepare 210 mL of saturated citric acid with 2% calcium chloride
- Add 4.2 grams of calcium chloride to 210 mL of water
- Add citric acid to saturation
- Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle
- Stir for 24hrs to allow solidification
- Rinse with water to remove excess reactants
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