User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/01/28: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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===Procedure=== | ===Procedure=== | ||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | ||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C | # Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | ||
# | #* 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) | ||
##* Add alginate '''while stirring''' PVA-water solution | ##* Add alginate '''while stirring''' PVA-water solution | ||
# | #* ~0.1 g of nanocomposites should be added to this mixture where applicable | ||
# Cool dissolved and mixed solution to 35°C | # Cool dissolved and mixed solution to 35°C | ||
# Prepare 210 mL of saturated citric acid with 2% calcium chloride | # Prepare 210 mL of saturated citric acid with 2% calcium chloride | ||
## Add 4.2 grams of calcium chloride to 210 mL of water | ## Add 4.2 grams of calcium chloride to 210 mL of water | ||
## Add citric acid to saturation | ## Add citric acid to saturation (32 g citric acid) | ||
# Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle | ##* 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid | ||
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a thin needle | |||
# Stir for 24hrs to allow solidification | # Stir for 24hrs to allow solidification | ||
# Rinse with water to remove excess reactants | # Rinse with water to remove excess reactants | ||
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**1.015 g PVA | **1.015 g PVA | ||
**0.1649 g alginate | **0.1649 g alginate | ||
*PVA-Alg #2 | |||
**0.998 g PVA | |||
**0.159 g Alg | |||
*PVA-Alg-NaMT | *PVA-Alg-NaMT | ||
**0.999 g PVA | **0.999 g PVA | ||
**0.161 g alginate | **0.161 g alginate | ||
**0.109 g NaMT | **0.109 g NaMT | ||
*Saturated citric acid, 2% calcium chloride solution | |||
**4.224 g (PVA-Alg), 4.231 g (PVA-Alg-NaMT), 4.207 g (PVA-Alg #2) calcium chloride | |||
**32.363 g (PVA-Alg), 32.330 g (PVA-Alg-NaMT), 32.267 g (PVA-Alg #2) citric acid | |||
====Notes==== | |||
*PVA-Alg beads pumped with syringe made fiber like clusters | |||
**not cooled at all | |||
**dropped in the middle | |||
*PVA-Alg-NaMT beads made with disposable dropper | |||
**cooled 20 seconds in dropper | |||
**dropped along side | |||
*Turn heat off but keep stirring to prevent clumping pre-bead forming | |||
*Let cool in dropper before dropping | |||
*Move to Dr Fox's automated pumper when repeating bead synthesis | |||
*Use wide dish | |||
*PVA-Alg #2 done in wide dish, dropped with disposable dropper, turn off heat ~1 min before dropping | |||
*Don't fill disposable dropper all the way > cooling solution will come out | |||
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Latest revision as of 00:41, 27 September 2017
PVA-Alg Bead Synthesis | Main project page Next entry |
January, 27, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>
Measurements
Notes
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