User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/01/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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*First trial of synthesizing PVA-Alginate beads with and without NaMT
*First trial of synthesizing PVA-Alginate beads with and without NaMT


==Protocol==
===Procedure===
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.>
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.>
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
## 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)  
#* 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)  
## ~0.1 g of nanocomposites should be added to this mixture where applicable
##* Add alginate '''while stirring''' PVA-water solution
#* ~0.1 g of nanocomposites should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of saturated citric acid with 2% calcium chloride
# Prepare 210 mL of saturated citric acid with 2% calcium chloride
## Add 4.2 grams of calcium chloride to 210 mL of water
## Add 4.2 grams of calcium chloride to 210 mL of water
## Add citric acid to saturation
## Add citric acid to saturation (32 g citric acid)
# Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle
##* 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a thin needle
# Stir for 24hrs to allow solidification
# Stir for 24hrs to allow solidification
# Rinse with water to remove excess reactants
# Rinse with water to remove excess reactants


====Measurements====
*PVA-Alg
**1.015 g PVA
**0.1649 g alginate
*PVA-Alg #2
**0.998 g PVA
**0.159 g Alg
*PVA-Alg-NaMT
**0.999 g PVA
**0.161 g alginate
**0.109 g NaMT
*Saturated citric acid, 2% calcium chloride solution
**4.224 g (PVA-Alg), 4.231 g (PVA-Alg-NaMT), 4.207 g (PVA-Alg #2) calcium chloride
**32.363 g (PVA-Alg), 32.330 g (PVA-Alg-NaMT), 32.267 g (PVA-Alg #2) citric acid
====Notes====
*PVA-Alg beads pumped with syringe made fiber like clusters
**not cooled at all
**dropped in the middle
*PVA-Alg-NaMT beads made with disposable dropper
**cooled 20 seconds in dropper
**dropped along side
*Turn heat off but keep stirring to prevent clumping pre-bead forming
*Let cool in dropper before dropping
*Move to Dr Fox's automated pumper when repeating bead synthesis
*Use wide dish
*PVA-Alg #2 done in wide dish, dropped with disposable dropper, turn off heat ~1 min before dropping
*Don't fill disposable dropper all the way > cooling solution will come out


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Latest revision as of 00:41, 27 September 2017

PVA-Alg Bead Synthesis Main project page
Next entry

January, 27, 2015

  • First trial of synthesizing PVA-Alginate beads with and without NaMT

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of nanocomposites should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 2% calcium chloride
    1. Add 4.2 grams of calcium chloride to 210 mL of water
    2. Add citric acid to saturation (32 g citric acid)
      • 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a thin needle
  5. Stir for 24hrs to allow solidification
  6. Rinse with water to remove excess reactants

Measurements

  • PVA-Alg
    • 1.015 g PVA
    • 0.1649 g alginate
  • PVA-Alg #2
    • 0.998 g PVA
    • 0.159 g Alg
  • PVA-Alg-NaMT
    • 0.999 g PVA
    • 0.161 g alginate
    • 0.109 g NaMT
  • Saturated citric acid, 2% calcium chloride solution
    • 4.224 g (PVA-Alg), 4.231 g (PVA-Alg-NaMT), 4.207 g (PVA-Alg #2) calcium chloride
    • 32.363 g (PVA-Alg), 32.330 g (PVA-Alg-NaMT), 32.267 g (PVA-Alg #2) citric acid

Notes

  • PVA-Alg beads pumped with syringe made fiber like clusters
    • not cooled at all
    • dropped in the middle
  • PVA-Alg-NaMT beads made with disposable dropper
    • cooled 20 seconds in dropper
    • dropped along side
  • Turn heat off but keep stirring to prevent clumping pre-bead forming
  • Let cool in dropper before dropping
  • Move to Dr Fox's automated pumper when repeating bead synthesis
  • Use wide dish
  • PVA-Alg #2 done in wide dish, dropped with disposable dropper, turn off heat ~1 min before dropping
  • Don't fill disposable dropper all the way > cooling solution will come out


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