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====R6G Fluorescence: Calibration and Measurement====
====R6G Fluorescence: Calibration and Measurement====
#Make stock concentrations (both groups can use the same solutions)
#Make stock concentrations (both groups can use the same solutions)
#*Initial stock: 2500uM, secondary stock: 500uM
#*0.10uM
#*0.10uM
#**Made with 500uM solution
#*0.50uM
#*0.50uM
#**Made with 500uM solution
#*1.0uM
#*1.0uM
#**Made with 500uM solution
#*1.5uM
#*1.5uM
#**Made with 500uM solution
#*2.0uM
#*2.0uM
#Take UV-Vis and Fluorescence spectra of these samples.
#**Made with 2500uM solution
#*0.75uM
#**Made with 500uM solution
#*1.2uM
#Take UV-Vis and Fluorescence spectra of these samples
#*Fluorimeter Settings:
#**500nm excitation
#**515-700nm scan range
#**10.0nm slit width
#**200nm/min scan speed
#Make a calibration curve based on UV-Vis.
#Make a calibration curve based on UV-Vis.
#*Compare your data to some published values
#*Compare your data to some published values
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===Data===
===Data===
====Calibration Curve: R6G Absorbance====
[[Image:2014_1001_R6G_calibration.PNG]]
====Measurement Results: R6G Absorbance====
[[Image:2014_1001_R6G_abs.PNG]]
====Calibration Curve: R6G Fluorescence====


====Measurement Results: R6G Fluoresence====
[[Image:2014_1001_R6G_fluor.PNG]]


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Revision as of 08:28, 1 October 2014

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October 1, 2014

Procedures

R6G Fluorescence: Calibration and Measurement

  1. Make stock concentrations (both groups can use the same solutions)
    • Initial stock: 2500uM, secondary stock: 500uM
    • 0.10uM
      • Made with 500uM solution
    • 0.50uM
      • Made with 500uM solution
    • 1.0uM
      • Made with 500uM solution
    • 1.5uM
      • Made with 500uM solution
    • 2.0uM
      • Made with 2500uM solution
    • 0.75uM
      • Made with 500uM solution
    • 1.2uM
  2. Take UV-Vis and Fluorescence spectra of these samples
    • Fluorimeter Settings:
      • 500nm excitation
      • 515-700nm scan range
      • 10.0nm slit width
      • 200nm/min scan speed
  3. Make a calibration curve based on UV-Vis.
    • Compare your data to some published values
  4. Make a calibration curve based on the fluorescence.
    • In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.

Data

Calibration Curve: R6G Absorbance

Measurement Results: R6G Absorbance

Calibration Curve: R6G Fluorescence

Measurement Results: R6G Fluoresence


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