User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/09/05

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September 5, 2014

Procedures

Completing Film Synthesis

Continued from Wednesday, 9/3/2014<br.> Procedure detailed by Dr Hartings

  • Stir in Sodium Sulfate, 1% HCl, and Sodium Bicarbonate solutions

Beginning New Film Synthesis

Procedure detailed by Dr Hartings

  • Polymer-10% Clay Film
    • 1.0004 g PVA and 0/1077 g sodium montmorillonite (~1g PVA and ~.1g NaM)
  • Polymer Film
    • 1.0050 g PVA (~1g)

UV-vis Measurements

  • Take UV Vis spectra for Malachite green at the following concentrations: 0ppm, 0.20ppm, 0.50ppm, 1.1ppm, 1.4ppm, 1.7ppm, and 2.0ppm
  • Construct calibration curve for each of the two absorbance maximums

<br.>

Equilibrium Measurements & Analysis

  • Dilute 200 ppm solutions 100x and 80 ppm solutions 50x to 10 mL & transfer to glass test tube (3 mL needed for absorbance measurements)

  • Determine the amount of Malachite green absorbed into a film
    • ε615=0.1293<br.>
    • A615=ε615bc → c=A615/ε615b:<br.>
    1. PVA film in 200 ppm
      • csoln=(0.092)/(0.1293)(1)= 0.716 ppm ×100= 71.6 ppm
    2. PVA film in 80 ppm
      • csoln=(0.094)/(0.1293)(1)= 0.727 ppm ×50= 36.35 ppm
    3. PVA film in 8 ppm
      • csoln=(0.064)/(0.1293)(1)= 0.495 ppm
    4. PVA film in 2 ppm
      • csoln=(0.009)/(0.1293)(1)= 0.070 ppm
    5. PVA Clay film in 200 ppm
      • csoln=(0.019)/(0.1293)(1)= 0.147 ppm ×100= 14.7 ppm
    6. PVA Clay film in 80 ppm
      • csoln=(0.010)/(0.1293)(1)= 0.077 ppm ×50= 3.85 ppm
    7. PVA Clay film in 8 ppm
      • csoln=(0.012)/(0.1293)(1)= 0.093 ppm
    8. PVA Clay film in 2 ppm
      • csoln=(0.004)/(0.1293)(1)= 0.031 ppm
    • ε425=0.0266<br.>
    • A425=ε425bc → c=A425/ε425b:<br.>
    1. PVA film in 200 ppm
      • csoln=(0.019)/(0.0266)(1)= 0.714 ppm ×100= 71.4 ppm
    2. PVA film in 80 ppm
      • csoln=(0.020)/(0.0266)(1)= 0.752 ppm ×50= 37.6 ppm
    3. PVA film in 8 ppm
      • csoln=(0.017)/(0.0266)(1)= 0.639 ppm
    4. PVA film in 2 ppm
      • csoln=(0.003)/(0.0266)(1)= 0.113 ppm
    5. PVA Clay film in 200 ppm
      • csoln=(0.005)/(0.0266)(1)= 0.188 ppm ×100= 18.8 ppm
    6. PVA Clay film in 80 ppm
      • csoln=(0.003)/(0.0266)(1)= 0.113 ppm ×50= 5.65 ppm
    7. PVA Clay film in 8 ppm
      • csoln=(0.006)/(0.0266)(1)= 0.226 ppm
    8. PVA Clay film in 2 ppm
      • csoln=(0.003)/(0.0266)(1)= 0.113 ppm
  • Determine the equilibrium of free Malachite green vs bound malachite green with I.C.E. table
Malachite Green
Initial Concentration mno
Change in Concentration vwx
Equilibrium Concentration
  • Determine the mass of malachite green absorbed per mass of film
    • PVA film in 200 ppm
      1. 615nm: 200 ppm - 71.6 ppm = 128.4 ppm MG absorbed
        • (128.4 mg MG/L soln)×(0.010 L soln)= 1.284 mg MG/film
        • 1.284 mg MG/ 98 mg film = 0.013 mg MG/mg film
      2. 425nm: 200 ppm- 71.4 ppm= 128.6 ppm MG absorbed
        • 1.286 mg MG/film
        • 1.286 mg MG/ 98 mg film = 0.013 mg MG/mg film
    • PVA film in 80 ppm
      1. 615nm: 80 ppm- 36.35 ppm = 43.65 ppm
        • 0.4365 mg MG/film
        • 0.4365 mg MG/ 89.3 mg= 0.005 mg MG/mg film
      2. 425nm: 80 ppm- 37.6 ppm = 42.4 ppm
        • 0.4240 mg MG/film
        • 0.4240 mg MG/ 89.3 mg= 0.005 mg MG/mg film
    • PVA film in 8 ppm
      1. 615nm: 8 ppm- 0.495 ppm = 7.505 ppm
        • 0.07505 mg MG/film
        • 0.07505 mg MG/ 58.7 mg= 0.0013 mg MG/mg film
      2. 425nm: 8 ppm- 0.639 ppm = 7.361 ppm
        • 0.07361 mg MG/film
        • 0.07361 mg MG/ 58.7 mg= 0.0013 mg MG/mg film
    • PVA film in 2 ppm
      1. 615nm: 2 ppm- 0.070 ppm= 1.93 ppm
        • 0.0193 mg MG/film
        • 0.0193 mg MG/ 128.6 mg= 0.00015 mg MG/mg film
      2. 425nm: 2 ppm- 0.113 ppm = 1.887 ppm
        • 0.0188 mg MG/film
        • 0.0188 mg MG/ 128.6 mg= 0.00015 mg MG/mg film
    • PVA Clay film in 200 ppm
      1. 615nm: 200 ppm - 14.7 ppm= 185.3 ppm
        • 1.853 mg MG/film
        • 1.853 mg MG/ 104.9 mg= 0.017 mg MG/mg film
      2. 425nm: 200 ppm - 18.8 ppm= 181.2 ppm
        • 1.812 mg MG/film
        • 1.812 mg MG/ 104.9 mg= 0.017 mg MG/mg film
    • PVA Clay film in 80 ppm
      1. 615nm: 80 ppm - 3.85 ppm= 76.15 ppm
        • 0.7615 mg MG/film
        • 0.7615 mg MG/ 76.5 mg= 0.010 mg MG/mg film
      2. 425nm: 80 ppm- 5.65 ppm= 74.35 ppm
        • 0.7435 mg MG/film
        • 0.7435 mg MG/ 76.5 mg= 0.010 mg MG/mg film
    • PVA Clay film in 8 ppm
      1. 615nm: 8 ppm- 0.093 ppm= 7.907 ppm
        • 0.07907 mg MG/film
        • 0.07907 mg MG/ 76.585 mg= 0.001 mg MG/mg film
      2. 425nm: 8 ppm- 0.226 ppm= 7.774 ppm
        • 0.07774 mg MG/film
        • 0.07774 mg MG/ 76.585 mg= 0.001 mg MG/mg film
    • PVA Clay film in 2 ppm
      1. 615nm: 2 ppm - 0.031 ppm= 1.969 ppm
        • 0.01969 mg MG/film
        • 0.01969 mg MG/ 68.9 mg= 0.00029 mg MG/mg film
      2. 425nm: 2 ppm - 0.113 ppm= 1.887 ppm
        • 0.01887 mg MG/film
        • 0.01887 mg MG/ 68.9 mg= 0.00027 mg MG/mg film




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