User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/22

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 20: Line 20:
*[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/1/celldetachmentprotocol.pdf Company procedure]
*[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/1/celldetachmentprotocol.pdf Company procedure]
 +
===Test Results===
 +
====Type I Collagen Test====
 +
 +
====Trypsin Cell Detachment Procedure & Cell Counting====
 +
*“Original” - 125 cells on scaffold
 +
*Polydopmaine & “original” - 450 cells on scaffold
 +
*Polydopmaine only- 150 cells on scaffold
 +
*Brute force - 300 cells on scaffold
 +
*Plain - 100 cells on scaffold
 +
 +
===Next time===
 +
*Perform all tests in triplicate

Revision as of 12:06, 22 April 2014

Cell Tests Main project page
Previous entry      Next entry

April 22, 2014

Objectives

  • Cell tests

Test Procedures

Type I Collagen Test (Detection and Quantification)

Collagen Staining Procedure & Microscope Imaging

Trypsin Cell Detachment Procedure & Cell Counting

Test Results

Type I Collagen Test

Trypsin Cell Detachment Procedure & Cell Counting

  • “Original” - 125 cells on scaffold
  • Polydopmaine & “original” - 450 cells on scaffold
  • Polydopmaine only- 150 cells on scaffold
  • Brute force - 300 cells on scaffold
  • Plain - 100 cells on scaffold

Next time

  • Perform all tests in triplicate



Personal tools