User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/22

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April 22, 2014

Objectives

  • Cell tests

Test Procedures

Type I Collagen Test (Detection and Quantification)

Collagen Staining Procedure & Microscope Imaging

Trypsin Cell Detachment Procedure & Cell Counting

Test Results

Type I Collagen Test

Trypsin Cell Detachment Procedure & Cell Counting

  • “Original” - 125 cells on scaffold
  • Polydopmaine & “original” - 450 cells on scaffold
  • Polydopmaine only- 150 cells on scaffold
  • Brute force - 300 cells on scaffold
  • Plain - 100 cells on scaffold

Next time

  • Perform all tests in triplicate