User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/08: Difference between revisions
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== | ==April 8, 2014== | ||
===Objectives=== | ===Objectives=== | ||
*Centrifuge 2nd procedure Ag:BSA NPs | *Centrifuge 2nd procedure Ag:BSA NPs | ||
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*Make Au nanofibers and print scaffolds (12) for brute force | *Make Au nanofibers and print scaffolds (12) for brute force | ||
===Sample Prep: Nanofibers for Brute Force=== | |||
[[Image:2014_0211_myo_bsa_NPs.PNG]] | |||
*Solutions: | |||
**Au: 0.011g in 10mL to make 2.793mM solution | |||
**Myoglobin: 0.011g in 10mL to make 0.0621mM solution | |||
====Brute Force Nanofiber-coated Scaffolds==== | |||
*4 PLA scaffolds printed for each test tube | |||
*Gold:Myoglobin (100:1) nanofibers synthesized by above method | |||
*Nanofibers dried onto PLA scaffolds | |||
*Scaffolds submerged in water | |||
*Autoclaved | |||
===Results: UV-vis=== | |||
[[Image:2014 0408 abs AgBSA NPs.PNG]] | |||
*Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm | |||
===Sample Prep: Scaffolds in Bacteria=== | |||
*Dry-autoclaved scaffolds submerged in bacteria solution | |||
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Revision as of 17:39, 18 April 2014
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April 8, 2014Objectives
Sample Prep: Nanofibers for Brute Force
Brute Force Nanofiber-coated Scaffolds
Results: UV-vis
Sample Prep: Scaffolds in Bacteria
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