User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Ag:BSA NPs</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==April 8, 2014== | ||
===Objectives=== | ===Objectives=== | ||
*Centrifuge 2nd procedure Ag:BSA NPs | *Centrifuge 2nd procedure Ag:BSA NPs | ||
* | *UVvis of Ag:BSA NPs from last time | ||
*Put dry-autoclaved scaffolds into bacteria | |||
*Make Au nanofibers and print scaffolds (12) for brute force | |||
===Sample Prep: Nanofibers for Brute Force=== | |||
[[Image:2014_0211_myo_bsa_NPs.PNG]] | |||
*Solutions: | |||
**Au: 0.011g in 10mL to make 2.793mM solution | |||
**Myoglobin: 0.011g in 10mL to make 0.0621mM solution | |||
====Brute Force Nanofiber-coated Scaffolds==== | |||
*4 PLA scaffolds printed for each test tube | |||
*Gold:Myoglobin (100:1) nanofibers synthesized by above method | |||
*Nanofibers dried onto PLA scaffolds | |||
*Scaffolds submerged in water | |||
*Autoclaved | |||
===Results: UV-vis=== | |||
[[Image:2014 0408 abs AgBSA NPs.PNG]] | |||
*Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm | |||
===Sample Prep: Scaffolds in Bacteria=== | |||
*Dry-autoclaved scaffolds submerged in bacteria solution | |||
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Revision as of 17:39, 18 April 2014
Ag:BSA NPs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 8, 2014Objectives
Sample Prep: Nanofibers for Brute Force
Brute Force Nanofiber-coated Scaffolds
Results: UV-vis
Sample Prep: Scaffolds in Bacteria
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