User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/02

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April 2, 2014

Objectives

  • Finish up Au:Myo Nanofiber scaffolds for cell culture
  • Make AgNPs with BSA according to article

Au:Myoglobin Nanofibers: Sample Prep for Cell Culture

  • Continued from yesterday
Finishing Up
  • If oven was not finished yesterday: submerge 3 pD scaffolds into original solution, leave other 3 in water and put in oven for 4 hours at 80C
  • Submerged dried brute force scaffolds in water
  • Autoclave all: 3 plain, 3 brute force, 3 original, 3 pD-Myo, 3 pD-Myo-Au

Sample Preparation: AgNPs

  • Continued from yesterday
With BSA (direct from article)



  • Cell tests

Type I Collagen Test (Detection and Quantification)

Collagen Staining Procedure & Microscope Imaging

Trypsin Cell Detachment Procedure & Cell Counting


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