User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> </span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Nanofiber-coated scaffolds and Ag:BSA fibers</span> | ||
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*From [http://www.sciencedirect.com/science/article/pii/S0021979712009526 Characterization of Silver/Bovine Serum Albumin (Ag/BSA) nanoparticles structure: Morphological, compositional, and interaction studies] | *From [http://www.sciencedirect.com/science/article/pii/S0021979712009526 Characterization of Silver/Bovine Serum Albumin (Ag/BSA) nanoparticles structure: Morphological, compositional, and interaction studies] | ||
*"Initially 0.9 g of BSA was mixed with 40 ml of deionized water. One milli liter of 0.423 M silver nitrate was added to BSA solution at ∼23 °C and sonicated for 10 min. This was followed by the addition of 1 ml of 0.423 M of sodium borohydride and sonicated for additional 30 min to reduce the silver ions in the solution. The resulting brown homogeneous solution was centrifuged at 21,500 rpm and the residue was washed with ethanol, suspended in water, and saved in an amber colored bottle at 4 °C." | *"Initially 0.9 g of BSA was mixed with 40 ml of deionized water. One milli liter of 0.423 M silver nitrate was added to BSA solution at ∼23 °C and sonicated for 10 min. This was followed by the addition of 1 ml of 0.423 M of sodium borohydride and sonicated for additional 30 min to reduce the silver ions in the solution. The resulting brown homogeneous solution was centrifuged at 21,500 rpm and the residue was washed with ethanol, suspended in water, and saved in an amber colored bottle at 4 °C." | ||
*Procedure from yesterday: | |||
Revision as of 12:01, 1 April 2014
 Nanofiber-coated scaffolds and Ag:BSA fibers
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April 2, 2014Objectives
Au:Myoglobin Nanofibers: Sample Prep for Cell Culture
Finishing Up
Sample Preparation: AgNPs
With BSA (direct from article)
Type I Collagen Test (Detection and Quantification)Collagen Staining Procedure & Microscope ImagingTrypsin Cell Detachment Procedure & Cell Counting | |
