User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/01: Difference between revisions

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===Objectives===
===Objectives===
*Making new scaffolds
*Making new scaffolds
===Au:Myoglobin Nanofibers: Sample Prep for Cell Culture===
[[Image:2014_0211_myo_bsa_NPs.PNG]]
*Solutions:
**Au: 0.011g in 10mL to make 2.793mM solution
**Myoglobin: 0.011g in 10mL to make 0.0621mM solution
**TRIS dopamine: 0.038g dopamine hydrochloride and 0.024g TRIS in 20mL to make 10mM solution at pH 8.46


====Plain Scaffolds====
*4 PLA scaffolds printed for each test tube
*Three test tubes filled with scaffolds and water
*Autoclaved
====Brute Force Nanofiber-coated Scaffolds====
*4 PLA scaffolds printed for each test tube
*Gold:Myoglobin (100:1) nanofibers synthesized by above method
*Nanofibers dried onto PLA scaffolds
*Scaffolds submerged in water
*Autoclaved
====Original Method for Nanofiber-coated Scaffolds====
*4 PLA scaffolds printed for each test tube
*Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with scaffolds in solution
*Autoclaved
====Polydopamine-coated Scaffolds====
*4 PLA scaffolds printed for each test tube
*Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour
*Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
*Rinse with deionized water and allow scaffold to dry overnight
*Scaffolds submerged in water
*Autoclaved
====Polydopamine Nanofiber-coated Scaffolds====
*4 PLA scaffolds printed for each test tube
*Scaffolds submerged in 10mM pH 8.5 TRIS dopamine hydrochloride solution for 1 hour
*Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
*Rinse with deionized water and allow scaffold to dry overnight
*Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution
*Autoclaved




Revision as of 16:46, 31 March 2014

Cell/Scaffold Tests <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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April 1, 2014

Objectives

  • Making new scaffolds

Au:Myoglobin Nanofibers: Sample Prep for Cell Culture

  • Solutions:
    • Au: 0.011g in 10mL to make 2.793mM solution
    • Myoglobin: 0.011g in 10mL to make 0.0621mM solution
    • TRIS dopamine: 0.038g dopamine hydrochloride and 0.024g TRIS in 20mL to make 10mM solution at pH 8.46

Plain Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Three test tubes filled with scaffolds and water
  • Autoclaved

Brute Force Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Gold:Myoglobin (100:1) nanofibers synthesized by above method
  • Nanofibers dried onto PLA scaffolds
  • Scaffolds submerged in water
  • Autoclaved

Original Method for Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with scaffolds in solution
  • Autoclaved

Polydopamine-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour
  • Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
  • Rinse with deionized water and allow scaffold to dry overnight
  • Scaffolds submerged in water
  • Autoclaved

Polydopamine Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Scaffolds submerged in 10mM pH 8.5 TRIS dopamine hydrochloride solution for 1 hour
  • Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
  • Rinse with deionized water and allow scaffold to dry overnight
  • Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution
  • Autoclaved




  • Cell tests

Type I Collagen Test (Detection and Quantification)

Collagen Staining Procedure & Microscope Imaging

Trypsin Cell Detachment Procedure & Cell Counting


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