User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/01: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
(5 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Scaffold Prep, and AgNPs without BSA</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
Line 9: | Line 9: | ||
===Objectives=== | ===Objectives=== | ||
*Making new scaffolds | *Making new scaffolds | ||
*New AgNP procedures | |||
===Au:Myoglobin Nanofibers: Sample Prep for Cell Culture=== | ===Au:Myoglobin Nanofibers: Sample Prep for Cell Culture=== | ||
[[Image:2014_0211_myo_bsa_NPs.PNG]] | [[Image:2014_0211_myo_bsa_NPs.PNG]] | ||
*Solutions: | *Solutions: | ||
**TRIS dopamine: 0. | **TRIS dopamine: 0.0475g dopamine hydrochloride and 0.03g TRIS in 25mL to make 10mM solution at pH 8.46 | ||
**Au: 0.011g in 10mL to make 2.793mM solution | **Au: 0.011g in 10mL to make 2.793mM solution | ||
**Myoglobin: 0.011g in 10mL to make 0.0621mM solution | **Myoglobin: 0.011g in 10mL to make 0.0621mM solution | ||
Line 50: | Line 51: | ||
===Breakdown=== | ===Breakdown=== | ||
=====Today===== | =====Today===== | ||
*Time sensitive: Make 40mL TRIS-polydopamine solution | *Time sensitive: Make 40mL TRIS-polydopamine solution | ||
*Time sensitive: Make 6 tests tubes of TRIS-polydopamine solution, put scaffolds in all 6 test tubes at '''12 p.m.''' (3 for pD only, 3 for pD-original) | *Time sensitive: Make 6 tests tubes of TRIS-polydopamine solution, put scaffolds in all 6 test tubes at '''12 p.m.''' (3 for pD only, 3 for pD-original) | ||
Line 66: | Line 67: | ||
*Autoclave all: 3 plain, 3 brute force, 3 original, 3 pD-Myo, 3 pD-Myo-Au | *Autoclave all: 3 plain, 3 brute force, 3 original, 3 pD-Myo, 3 pD-Myo-Au | ||
===Sample Preparation: AgNPs=== | |||
=====Without BSA===== | |||
*From [http://pubs.acs.org/doi/pdf/10.1021/ed084p322 Synthesis and Study of Silver Nanoparticles] | |||
*10 ml of 1 mM AgNO3 was added dropwise (about 1 drop/sec) to 30 ml of 2 mM NaBH4 (cooled in ice bath), while the reaction mixture was vigorously stirred using a magnetic stir plate. Entire procedure takes about 3 minutes. | |||
===Sample Preparation: Bacteria Culture=== | |||
*Make solution | |||
*Autoclave | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 11:05, 1 April 2014
Scaffold Prep, and AgNPs without BSA | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 1, 2014Objectives
Au:Myoglobin Nanofibers: Sample Prep for Cell Culture
Plain Scaffolds
Brute Force Nanofiber-coated Scaffolds
Original Method for Nanofiber-coated Scaffolds
Polydopamine-coated Scaffolds
Polydopamine Nanofiber-coated Scaffolds
BreakdownToday
Tomorrow
Sample Preparation: AgNPsWithout BSA
Sample Preparation: Bacteria Culture
|