User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/19

From OpenWetWare
Jump to navigationJump to search
Polydopamine Myoglobin Nanofibers, Silver Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 19, 2014

Objectives

  • Finish polydopamine nanofiber scaffolds
  • Wet-autoclave nanofiber coated scaffold in PBS
  • Attempt to make silver:protein nanofibers

Sample Preparation: Polydopamine nanofibers

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, protein, and water for each test tube (Myoglobin ratios from Febrary 11 pictured below):
    • Final [Au]=0.5mM, 5mL total volume

Autoclaved Nanofiber-coated Scaffold

  • Nanofiber coated scaffold from February 5 wet-autoclaved in PBS
    • Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
    • This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS

Macro image:<br.> <br.> Micro images:<br.> <br.> <br.>

Sample Preparation: Silver:BSA Nanofiber

  • We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
  • The final solution will have 0.25mM AgNO3, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
  • All samples will be heated to 80C in an oven for 4 hours



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/19&oldid=774709"