User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/19

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(Sample Preparation: Polydopamine nanofibers)
 
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*Myoglobin stock solution:
*Myoglobin stock solution:
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
-
*Volumes of Au, protein, and water for each test tube (Myoglobin ratios from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation Febrary 11]):
+
*Volumes of Au, protein, and water for each test tube (Myoglobin ratios from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation Febrary 11] pictured below):
**Final [Au]=0.5mM, 5mL total volume
**Final [Au]=0.5mM, 5mL total volume
**[[Image:2014_0211_myo_bsa_NPs.PNG]]
**[[Image:2014_0211_myo_bsa_NPs.PNG]]

Current revision

Polydopamine Myoglobin Nanofibers, Silver Nanofibers Main project page
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February 19, 2014

Objectives

  • Finish polydopamine nanofiber scaffolds
  • Wet-autoclave nanofiber coated scaffold in PBS
  • Attempt to make silver:protein nanofibers

Sample Preparation: Polydopamine nanofibers

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, protein, and water for each test tube (Myoglobin ratios from Febrary 11 pictured below):
    • Final [Au]=0.5mM, 5mL total volume
    • Image:2014_0211_myo_bsa_NPs.PNG

Autoclaved Nanofiber-coated Scaffold

  • Nanofiber coated scaffold from February 5 wet-autoclaved in PBS
    • Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
    • This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS

Macro image:

Micro images:


Sample Preparation: Silver:BSA Nanofiber

  • We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
  • The final solution will have 0.25mM AgNO3, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
  • All samples will be heated to 80C in an oven for 4 hours



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