(Difference between revisions)
 Revision as of 12:38, 19 February 2014 (view source)← Previous diff Revision as of 12:39, 19 February 2014 (view source)Next diff → Line 18: Line 18: *Myoglobin stock solution: *Myoglobin stock solution: **0.011g x (1 mol/17699g) x 0.010L = 0.0621mM **0.011g x (1 mol/17699g) x 0.010L = 0.0621mM - *Volumes of Au, protein, and water for each test tube (from [User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation|February 11, 2014]: + *Volumes of Au, protein, and water for each test tube (from [February 11, 2014 User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation]: **Final [Au]=0.5mM, 5mL total volume **Final [Au]=0.5mM, 5mL total volume **[[Image:2014_0211_myo_bsa_NPs.PNG]] **[[Image:2014_0211_myo_bsa_NPs.PNG]]

## Revision as of 12:39, 19 February 2014

Polydopamine Myoglobin Nanofibers Main project page
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## February 19. 2014

### Objectives

• Finish polydopamine nanofiber scaffolds

### Procedures

• Polydopamine nanofibers
• Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
• Prepare nanofibers according to previously used procedure, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios of gold:myoglobin

### Sample Preparation

• Au stock solution:
• 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
• Myoglobin stock solution:
• 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
• Volumes of Au, protein, and water for each test tube (from [February 11, 2014 User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation]:
• Final [Au]=0.5mM, 5mL total volume