User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/19: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Polydopamine Myoglobin Nanofibers</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Polydopamine Myoglobin Nanofibers, Silver Nanofibers</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==February 19. 2014==
==February 19, 2014==
===Objectives===
===Objectives===
*Finish polydopamine nanofiber scaffolds
*Finish polydopamine nanofiber scaffolds
===Procedures===
*Wet-autoclave nanofiber coated scaffold in PBS
*Polydopamine nanofibers
*Attempt to make silver:protein nanofibers
**Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
**Prepare nanofibers according to previously used procedure, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios of gold:myoglobin
**Make an additional solution of nanofibers with no scaffold for comparison
*Autoclave scaffold in PBS from February 5
**Pictures before and after


===Sample Preparation===
===Sample Preparation: Polydopamine nanofibers===
*Au stock solution:
*Au stock solution:
**0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
**0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
*Myoglobin stock solution:
*Myoglobin stock solution:
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
*Volumes of Au, protein, and water for each test tube (Myoglobin ratios from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation Febrary 11]):
*Volumes of Au, protein, and water for each test tube (Myoglobin ratios from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation Febrary 11] pictured below):
**Final [Au]=0.5mM, 5mL total volume
**Final [Au]=0.5mM, 5mL total volume
**[[Image:2014_0211_myo_bsa_NPs.PNG]]
**[[Image:2014_0211_myo_bsa_NPs.PNG]]
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===Autoclaved Nanofiber-coated Scaffold===
===Autoclaved Nanofiber-coated Scaffold===
*Nanofiber coated scaffold from February 5 wet-autoclaved in PBS  
*Nanofiber coated scaffold from February 5 wet-autoclaved in PBS  
**Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
**This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS
Macro image:<br.>
[[Image:Photo_Feb_19,_2_11_50_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_2_11_50_PM.jpg|300px]]<br.>
Micro images:<br.>
[[Image:Test1PBS_autoclav_myo10x.jpg|400px]]<br.>
[[Image:Test1PBS_autoclav_myo10x3.jpg|400px]]<br.>
===Sample Preparation: Silver:BSA Nanofiber===
*We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
*The final solution will have 0.25mM AgNO<sub>3</sub>, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
*All samples will be heated to 80C in an oven for 4 hours
[[Image:2014_0219_Ag_BSA_nanofibers_ratios.PNG|900px]]


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Revision as of 13:31, 19 February 2014

Polydopamine Myoglobin Nanofibers, Silver Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 19, 2014

Objectives

  • Finish polydopamine nanofiber scaffolds
  • Wet-autoclave nanofiber coated scaffold in PBS
  • Attempt to make silver:protein nanofibers

Sample Preparation: Polydopamine nanofibers

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, protein, and water for each test tube (Myoglobin ratios from Febrary 11 pictured below):
    • Final [Au]=0.5mM, 5mL total volume

Autoclaved Nanofiber-coated Scaffold

  • Nanofiber coated scaffold from February 5 wet-autoclaved in PBS
    • Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
    • This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS

Macro image:<br.> <br.> Micro images:<br.> <br.> <br.>

Sample Preparation: Silver:BSA Nanofiber

  • We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
  • The final solution will have 0.25mM AgNO3, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
  • All samples will be heated to 80C in an oven for 4 hours



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