User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/18

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**Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
**Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
**Rinse with deionized water and allow scaffold to dry overnight
**Rinse with deionized water and allow scaffold to dry overnight
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===Observations===
 +
*Brute force
 +
 +
*Scaffolds after 1 hr in Tris-polydopamine
 +
 +
*Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution

Revision as of 14:44, 18 February 2014

Polydopamine Myoglobin Nanofibers Main project page
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February 18, 2014

Objectives

  • Polydopamine procedure for nanofibers
  • Myoglobin procedure for nanofibers (repeat)

Procedures

  • Preparing scaffolds
    • Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
      • First two scaffolds immersed in solution pH 8.51
      • Second two scaffolds immersed in solution pH 8.59
    • Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
    • Rinse with deionized water and allow scaffold to dry overnight

Observations

  • Brute force
  • Scaffolds after 1 hr in Tris-polydopamine
  • Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution



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