User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/18: Difference between revisions

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==February 18, 2014==
==February 18, 2014==
===Objectives===
===Objectives===
*Polydopamine procedure
*Polydopamine procedure for nanofibers
*Myoglobin procedure (repeat)
*Myoglobin procedure for nanofibers (repeat)
===Procedures===
===Procedures===
[Tuesday]
[Tuesday]

Revision as of 12:59, 12 February 2014

Polydopamine Myoglobin Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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February 18, 2014

Objectives

  • Polydopamine procedure for nanofibers
  • Myoglobin procedure for nanofibers (repeat)

Procedures

[Tuesday]

  • Polydopamine nanofibers
    • Immerse PLA scaffolds in 10mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
    • Add (10mM?) protein to solution for 5 hours to allow surface functionalization to occur
    • Rinse with deionized water and allow scaffold to dry overnight

[Wednesday]

  • Polydopamine nanofibers cont'd
    • Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
  • Myoglobin nanofibers
    • Same as usual, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios again



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