User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/18: Difference between revisions
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*Myoglobin procedure for nanofibers (repeat) | *Myoglobin procedure for nanofibers (repeat) | ||
===Procedures=== | ===Procedures=== | ||
*Preparing scaffolds | |||
* | **Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour | ||
**Immerse PLA scaffolds in | ***First two scaffolds immersed in solution pH 8.51 | ||
**Add | ***Second two scaffolds immersed in solution pH 8.59 | ||
**Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours | |||
**Rinse with deionized water and allow scaffold to dry overnight | **Rinse with deionized water and allow scaffold to dry overnight | ||
[ | ===Observations=== | ||
*Brute force | |||
* | [[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]] [[Image:Bforce_myo.jpg|300px]]<br.> | ||
[[Image:Bforce_myo_10x3.jpg|300px]] [[Image:Bforce_myo3.jpg|300px]]<br.> | |||
* | *Scaffolds after 1 hr in Tris-polydopamine | ||
[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]] | |||
*Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution | |||
Revision as of 12:09, 18 February 2014
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February 18, 2014Objectives
Procedures
Observations
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