User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/18: Difference between revisions
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*Myoglobin procedure for nanofibers (repeat) | *Myoglobin procedure for nanofibers (repeat) | ||
===Procedures=== | ===Procedures=== | ||
*Preparing scaffolds | |||
* | |||
**Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour | **Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour | ||
**Add | ***First two scaffolds immersed in solution pH 8.51 | ||
***Second two scaffolds immersed in solution pH 8.59 | |||
**Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours | |||
**Rinse with deionized water and allow scaffold to dry overnight | **Rinse with deionized water and allow scaffold to dry overnight | ||
Revision as of 11:37, 18 February 2014
 Polydopamine Myoglobin Nanofibers
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February 18, 2014Objectives
Procedures
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