User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12

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*160:1 BSA
*160:1 BSA
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*120:1 BSA
*120:1 BSA
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*80:1 BSA
*80:1 BSA
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*60:1 Myoglobin
*60:1 Myoglobin
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===Autoclaved Scaffolds===
===Autoclaved Scaffolds===
*PLA scaffolds were dry autoclaved
*PLA scaffolds were dry autoclaved

Revision as of 16:10, 19 February 2014

Myoglobin and BSA Nanofibers Main project page
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February 12, 2013

Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA

  • 120:1 BSA

  • 80:1 BSA

  • 100:1 Myoglobin

  • 60:1 Myoglobin

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid

Nanofiber Retention Test Results (February 19, 2014)

  • 160:1 BSA



  • 120:1 BSA



  • 80:1 BSA



  • 60:1 Myoglobin



Autoclaved Scaffolds

  • PLA scaffolds were dry autoclaved
  • Scaffolds did not melt as expected


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