User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12: Difference between revisions

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#PBS
#PBS
#strong acid
#strong acid
====Nanofiber Retention Test Results (February 19, 2014)====
*160:1 BSA
[[Image:Photo_Feb_19,_2_59_58_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_3_00_06_PM.jpg|300px]]
*120:1 BSA
[[Image:Photo_Feb_19%2C_2_57_38_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_2_57_50_PM.jpg|300px]]
*80:1 BSA
[[Image:Photo_Feb_19,_2_58_48_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_2_58_54_PM.jpg|300px]]
*60:1 Myoglobin
[[Image:Photo_Feb_19,_2_56_39_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_2_56_50_PM.jpg|300px]]
===Autoclaved Scaffolds===
*PLA scaffolds were dry autoclaved
*Scaffolds did not melt as expected
[[Image:Photo_Feb_12,_2_49_20_PM.jpg|300px]]


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Revision as of 13:09, 19 February 2014

Myoglobin and BSA Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 12, 2013

Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA

  • 120:1 BSA

  • 80:1 BSA

  • 100:1 Myoglobin

  • 60:1 Myoglobin

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid

Nanofiber Retention Test Results (February 19, 2014)

  • 160:1 BSA

<br.>

  • 120:1 BSA

<br.>

  • 80:1 BSA

<br.>

  • 60:1 Myoglobin

<br.>

Autoclaved Scaffolds

  • PLA scaffolds were dry autoclaved
  • Scaffolds did not melt as expected


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