User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12: Difference between revisions

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*Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
*Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
*Brute force method with 0.5mM gold-Myoglobin nanofibers
*Brute force method with 0.5mM gold-Myoglobin nanofibers
*Autoclave one dry, one wet scaffold
===Myoglobin and BSA Nanofiber Results===
===Myoglobin and BSA Nanofiber Results===
*160:1 BSA
*160:1 BSA

Revision as of 10:31, 12 February 2014

Myoglobin and BSA Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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February 12, 2013

Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA
  • 120:1 BSA
  • 80:1 BSA
  • 100:1 Myoglobin
  • 60:1 Myoglobin

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid