User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/12: Difference between revisions
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== | ==November 12, 2013== | ||
===Objective=== | ===Objective=== | ||
#Atomic Absorption of nanoparticles | |||
#UV-vis measurements of Lysozyme and Myoglobin nanoparticles | |||
===Description=== | |||
<u>Atomic Absorption:</u><br.> | |||
*Centrifuge 4mL from each sample for 30min at 4000rpm | |||
*Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL | |||
*Take measurements of your standards and samples | |||
*Make a graph showing concentration of Au vs Au:protein ratio | |||
=====Gold Standards- Calculations===== | |||
#50 ug/mL: | |||
#*(1000ug/mL)V=(50ug/mL)(5mL) | |||
#*V= 0.25mL Au stock in 4.75mL water | |||
*Each sample diluted from Au stock in the above manner | |||
===Data=== | ===Data=== |
Revision as of 09:53, 12 November 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
November 12, 2013Objective
DescriptionAtomic Absorption:<br.>
Gold Standards- Calculations
DataNotes |