User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/23

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(Description)
(Description)
Line 31: Line 31:
*Take UV-vis spectrum of each ratio sample
*Take UV-vis spectrum of each ratio sample
*Save the sample
*Save the sample
-
=====HRP-NP Synthesis=====
+
=====HRP-AuNP Synthesis=====
Ratios:
Ratios:
#230
#230
Line 43: Line 43:
#390
#390
#410
#410
 +
=====Hemoglobin-AuNPs=====
 +
Ratios:
 +
# 30
 +
# 50
 +
# 70
 +
# 90
 +
# 110
 +
# 130
 +
# 150
 +
# 170
 +
# 190
 +
# 210
===Data===
===Data===

Revision as of 11:23, 23 October 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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October 23, 2013

Happy Mole Day!

Objective

To measure the activity of pepsin in solution and pepsin-AuNPs in solution.

Description

Pepsin-AuNP Analysis

(picture)
Physical Characteristics (by ratio):

  1. 30-
  2. 50-
  3. 70-
  4. 90-
  5. 110-
  6. 130-
  7. 150-
  8. 170-
  9. 190-
  10. 210-


  • Centrifuge 1mL of each ratio of NPs for 30 minutes
  • Take UV-vis spectrum of each ratio sample
  • Save the sample
HRP-AuNP Synthesis

Ratios:

  1. 230
  2. 250
  3. 270
  4. 290
  5. 310
  6. 330
  7. 350
  8. 370
  9. 390
  10. 410
Hemoglobin-AuNPs

Ratios:

  1. 30
  2. 50
  3. 70
  4. 90
  5. 110
  6. 130
  7. 150
  8. 170
  9. 190
  10. 210

Data

Notes


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