User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/23: Difference between revisions
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===Description=== | ===Description=== | ||
*Make a 37°C water bath to hold at least 3 test tubes (same size as yesterday) | *Make a 37°C water bath to hold at least 3 test tubes (same size as yesterday) | ||
====Pepsin Digest==== | =====Pepsin Digest===== | ||
#Make 3 1mL dilutions for regular pepsin in 0.01N HCl at the following concentrations: | #Make 3 1mL dilutions for regular pepsin in 0.01N HCl at the following concentrations: | ||
#* 10μg/mL | #* 10μg/mL | ||
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#Calculate the number of units/mg of active enzyme in each sample | #Calculate the number of units/mg of active enzyme in each sample | ||
#*Units/mg = [A<sub>280</sub>(Test) - A<sub>280</sub>(Blank)]*1000/[10minutes*mg enzyme in reaction mixture] | #*Units/mg = [A<sub>280</sub>(Test) - A<sub>280</sub>(Blank)]*1000/[10minutes*mg enzyme in reaction mixture] | ||
====Pepsin-AuNP Analysis==== | ====Pepsin-AuNP Analysis==== | ||
(picture) | (picture) |
Revision as of 08:44, 23 October 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
October 23, 2013Happy Mole Day! ObjectiveTo measure the activity of pepsin in solution and pepsin-AuNPs in solution. Description
Pepsin Digest
Pepsin-AuNP Analysis(picture) Physical Characteristics (by ratio):<br.>
<br.>
DataNotes |