User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/16

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===Description===
===Description===
-
+
<u>Reactions (Amounts in Cuvette)</u>
 +
*Luminol: final concentration so A(330)<1
 +
**Stock: 1.46mM
 +
**Make 20x dilution
 +
**Final: 0.073mM
 +
*H<sub>2</sub>O<sub>2</sub>: final concentration 50x [Luminol]
 +
**Stock: 44.29mM
 +
**50×0.073mM=3.65
 +
**Final: 3.65mM
 +
*HRP-NPs: use 100uL of 60:1 solution
 +
**[Au]=0.25mM
 +
**[HRP]=4.167uM
===Data===
===Data===

Revision as of 13:08, 16 October 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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October 16, 2013

Objective

  • Test the activity of HRP-AuNPs today for the catalytic conversion of luminol

Description

Reactions (Amounts in Cuvette)

  • Luminol: final concentration so A(330)<1
    • Stock: 1.46mM
    • Make 20x dilution
    • Final: 0.073mM
  • H2O2: final concentration 50x [Luminol]
    • Stock: 44.29mM
    • 50×0.073mM=3.65
    • Final: 3.65mM
  • HRP-NPs: use 100uL of 60:1 solution
    • [Au]=0.25mM
    • [HRP]=4.167uM

Data

Notes


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