User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/01: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 29: Line 29:
[[Image:2013_1001_beer's_law_calcs.PNG]]<br.>
[[Image:2013_1001_beer's_law_calcs.PNG]]<br.>


<u>Kinetic Measurements</u>




===Notes===


[[Category:]]
[[Category:Miscellaneous]]




Revision as of 09:13, 2 October 2013

Experimental Biological Chemistry: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


October 1, 2013

Objective

  1. UV-Vis Absorbance of reactants, catalysts, and products
    • Make 10mL stock solution of luminol at appropriate dilution
  2. Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer

Description

UV-vis Samples<br.>

  • Blank: TRIS buffer
  • Horseradish peroxidase
  • H2O2
  • 20x luminol
  • 10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H2O2 catalyzed by 333uL HRP)

Data

Absorbance Measurements<br.> <br.> Beer's Law concentration calculations<br.> <br.>

Kinetic Measurements


Notes


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/CHEM-571_2013F/2013/10/01&oldid=734375"