User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/04

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==September 4,2013==
==September 4,2013==
===Objective===
===Objective===
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Execute the following [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/04 procedure] examining protein fluoresecence.
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Continue procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-571_2013F/2013/09/03 yesterday].
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===Procedure===
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*Selected protein:
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===Data===
===Data===
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====Absorbance Measurements====
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====Absorption Spectra====
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[[Image:2013_0904_inosine_spectra.PNG]]<br.>
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All peaks at 249nm. <br.>
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====Fluorescence Measurements====
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===Calculations and Analysis===
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====Calibration Curves====
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[[Image:2013_0904_inosine_calibration.PNG]]
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===Analysis===
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=====Determining Unknown's Concentration=====
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====Integration====
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*Unknown from:
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*Concentration determined from calibration curve:
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*Actual concentration:
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====Normalization====
 

Revision as of 13:22, 4 September 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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September 4,2013

Objective

Continue procedure from yesterday.

Data

Absorption Spectra

Image:2013_0904_inosine_spectra.PNG
All peaks at 249nm.

Calculations and Analysis

Calibration Curves

Image:2013_0904_inosine_calibration.PNG

Determining Unknown's Concentration
  • Unknown from:
  • Concentration determined from calibration curve:
  • Actual concentration:





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