User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/04: Difference between revisions

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==September 4,2013==
==September 4,2013==
===Objective===
===Objective===
Execute the following [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/04 procedure] examining protein fluoresecence.
Continue procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-571_2013F/2013/09/03 yesterday].
===Procedure===
*Selected protein:
 


===Data===
===Data===
====Absorbance Measurements====
====Absorption Spectra====
[[Image:2013_0904_inosine_spectra.PNG]]<br.>
All peaks at 249nm. <br.>


====Fluorescence Measurements====
===Calculations and Analysis===
====Calibration Curves====
[[Image:2013_0904_inosine_calibration.PNG]]


===Analysis===
=====Determining Unknown's Concentration=====
====Integration====
*Unknown from:
*Concentration determined from calibration curve:
*Actual concentration:


====Normalization====




Revision as of 10:22, 4 September 2013

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September 4,2013

Objective

Continue procedure from yesterday.

Data

Absorption Spectra

<br.> All peaks at 249nm. <br.>

Calculations and Analysis

Calibration Curves

Determining Unknown's Concentration
  • Unknown from:
  • Concentration determined from calibration curve:
  • Actual concentration:





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